Photoactive Ru(II) -polypyridyl complexes that display sequence selectivity and high-affinity binding to duplex DNA through groove binding |
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Authors: | Ghosh Amrita Das Priyadip Gill Martin R Kar Prasenjit Walker Michael G Thomas Jim A Das Amitava |
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Affiliation: | Central Salt & Marine Chemicals Research Institute, Bhavnagar, 364002 Gujarat, India. |
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Abstract: | The duplex-DNA binding properties of a nonintercalating polypyridyl ruthenium(II) complex that incorporates a linear extended ligand with a catechol moiety has been probed with a variety of photo- and biophysical techniques. These studies reveal that the complex groove binds to DNA sequences biphasically, and displays binding constants equivalent to those of high-affinity metallointercalators. The complex also displays preferential binding to AT-rich sequences. Changes in the structure of the coordinated catechol ligand and the incorporation of intercalating ancillary ligands into the complex were found to modulate both the optical-binding response and binding parameters of the system, which indicates that the catechol moiety plays a crucial role in the observed enhancement to binding affinities. |
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Keywords: | DNA groove binding luminescence molecular recognition ruthenium |
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