Determination of the epimerization rate constant of amygdalin by microemulsion electrokinetic chromatography |
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Authors: | Yu Lishuang Ye Hongzhi Zheng Lili Chen Lidian Chu Kedan Liu Xianxiang Xu Xueqin Chen Guonan |
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Affiliation: | College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, P. R. China. |
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Abstract: | A new method for separation and determination of amygdalin and its epimer (neoamygdalin) in the epimerization of amygdalin by MEEKC is proposed. For the chiral separation of amygdalin and neoamygdalin, a running buffer composed of 80 mM sodium cholate, 5.0% v/v butan‐1‐ol, 0.5% v/v heptane and 94.5% v/v 30 mM Na2B4O7 buffer (pH 9.00) is proposed. Under optimum conditions, the basic separation of amygdalin and neoamygdalin can be achieved within 7 min. The calibration curve for amygdalin showed excellent linearity in the concentration range of 20–1000 μg/mL with a detection limit of 5.0 μg/mL (S/N=3). The epimerization rate constant of amygdalin in basic microemulsion was first determined by monitoring the concentration changes of amygdalin, and the epimerization rate constant of amygdalin was found to be 2×10?3 min?1 at 25°C under the above optimum microemulsion conditions. |
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Keywords: | Amygdalin Electrokinetic chromatography Epimerization rate constant Microemulsion |
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