盐酸吡哆辛在聚L-色氨酸修饰电极上的电化学行为及其分析测定

制备了聚L-色氨酸修饰玻碳电极(PTRP/GCE)，用循环伏安法、线性单扫描伏安法、计时电量法等研究了盐酸吡哆辛(VB6)在PTRP/GCE上的电化学行为及电化学动力学性质， 实验表明：VB6在PTRP/GCE上的电极过程为1电子1质子的不可逆氧化反应，在20～400mV/s范围内，峰电流与扫速的平方根呈良好的线性关系，电极活化面积A为0.29cm2，扩散系数D为1.9612×10-4cm2/s。在pH=3的HAc-NaAc缓冲溶液中，VB6在PTRP/GCE电极上氧化峰电流与其浓度在1×10-4～5×10-6mol/L范围内呈良好的线性关系，线性回归方程为：ipa（μA） =7.7399+408.8129c (mmol/L)，R=0.9931，检出限为1×10-6mol/L，VB6样品测定平均回收率为100.15%。

Electrochemical Behavior and Determination of VitaminB6 at poly(L-tryptophan) Modified Electrode

poly (L-tryptophan) modified glassy carbon electrode (PTRP/GCE) was prepared, Electrochemical behaviors and kinetic parameters of VB6 were investigated at PTRP/GCE by cyclic voltammetry(CV) , Linear Sweep Voltammetry(LSV), chronoamperometry (CA) and chronocoulometry (CC). Electrochemical results show that VB6 electrochemical behaviors exhibit a irreversible reaction at 1.085V(vs.SCE) with the electron transfer number of 1 and the proton number of 1. The peak currents are linearly related to the square root of scan rates in the scan rates range of 20～400mV/s .The effective area of PTRP/GCE Aeff and the diffusion coefficient D are 0.29cm2 and 1.9612×10-4cm2/s, respectively. In a HAc-NaAc buffer solution of pH =3.0, the oxidation peak currents of VB6 versus its concentration have a good linear relationship in the concentration range of 1×10-4～5×10-6mol/L with the correlation coefficient of 0.9931 and the detection limit of 1×10-6mol/L. The linear regress equation was ipa=7.7399+408.8129c (ipa：μA，c：mmol/L) and the recovery is 100.15%