| 吖啶橙(AO)-藏红T(ST)荧光探针测定DNA的研究 |
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| 引用本文： | 陈鸿琪,李光源. 吖啶橙(AO)-藏红T(ST)荧光探针测定DNA的研究[J]. 理化检验(化学分册), 2000, 36(9): 385-387,390 |
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| 作者姓名： | 陈鸿琪 李光源 |
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| 作者单位： | 1. 山东临沂师范学院,临沂276001
2. 山东临沂广播电视大学 |
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| 摘 要： | 简述了以荧光能量转移本系测定ＤＮＡ方法的发展过程，同时对吖啶（ＡＯ）－藏红Ｔ（ＳＴ）荧光探针测定ＤＮＡ的条件进行分析，证明了该法灵敏度高、线性范围宽、检出限低、选择性好、抗干扰能力强，该法为微曦ＤＮＡ测定分析工作提供了一个新的途径。
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| 关 键 词： | 荧光探针 吖啶橙（AO）－藏红T（ST） DNA测定 |
STUDY ON THE DETERMINATION OF DNA USING ACRIDINE ORANGE-SAFRANINE T AS FLUORESCENCE PROBE |
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| Chen Honqi,Li Guangyuan. STUDY ON THE DETERMINATION OF DNA USING ACRIDINE ORANGE-SAFRANINE T AS FLUORESCENCE PROBE[J]. Physical Testing and Chemical Analysis Part B:Chemical Analgsis, 2000, 36(9): 385-387,390 |
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| Authors: | Chen Honqi Li Guangyuan |
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| Affiliation: | Chen Honqi;(Shandong Linyi Teachers College Linyi 276001); Li Guangyuan;(Shandong Linyi Television and Broadcasting University) |
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| Abstract: | A brief review on the recent progress of the determination of DNA using the fluorescence energy transfer system, and a thorough study on the optimum conditions for the determination of DNA using acridine orange safranine T reaction system as fluorescence probe is given in this paper. It is proved that the above mentioned method has the predominant advantages of high sensitivity and selectivity, low detection limit and wide range of linearity, and that it is a new and preferable approach for the determination of DNA. |
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| Keywords: | Fluorescence energy transfer Fluorescence probe Acridine orange-safranine T |
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