Synthesis and testing of chromogenic phenoxazinone substrates for beta-alanyl aminopeptidase |
| |
Authors: | Zaytsev Andrey V Anderson Rosaleen J Bedernjak Alexandre Groundwater Paul W Huang Yongxue Perry John D Orenga Sylvain Roger-Dalbert Celine James Arthur |
| |
Affiliation: | Pharmacy, Chemistry and Biomedical Sciences, University of Sunderland, Sunderland, SR1 3SD, U.K. |
| |
Abstract: | Novel 7-N-(beta-alanyl)aminophenoxazin-3-one salts 27a-d have been synthesized and tested as chromogenic substrates for beta-alanyl aminopeptidase, which is present in Pseudomonas aeruginosa, the most common respiratory pathogen in patients with cystic fibrosis. The biological results show that 7-N-(beta-alanyl)amino-1-pentylphenoxazin-3-one trifluoroacetate salt 27a is a chromogenic substrate for this bacterium, with a low degree of diffusion in nutrient media for growing bacterial cultures and a bright red colour, making it easily distinguishable from the agar background. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|