|
|||||
|
|
| 基体辅助激光解吸电离飞行时间质谱法研究蛋白质的标记反应 | |
| 引用本文： | 韦寿莲,邓光辉,郑一宁,莫金垣. 基体辅助激光解吸电离飞行时间质谱法研究蛋白质的标记反应[J]. 分析化学, 2002, 30(2): 162-164 |
| 作者姓名： | 韦寿莲 邓光辉 郑一宁 莫金垣 |
| 作者单位： | 1. 肇庆学院轻化系,肇庆,526061;广西民族学院化学化工系,南宁,530006 2. 广西民族学院化学化工系,南宁,530006 3. 中山大学化学化工学院,广州510275 |
| 基金项目： | 广西壮族自治区自然科学基金资助项目（No．9912001） |
| 摘 要： | 利用基体辅助激光解吸电离飞行时间质谱法研究了商品化的生物素和异硫氰荧光素（FITC）标记和胰岛素的组成。其后利用基体辅助激光解吸电离飞行时间质谱法在位监控了生物素标记胰岛素的反应。结果发现：（1）商品化的标记胰岛素是一个多种标记比共存的混合物，而且两种商品化的标记胰岛素均包括了未标记的胰岛素；（2）标记物与蛋白质的摩尔比是决定标记物组成的重要因素，过高浓度标记物的使用有可能导致一些异常组份的生成。 |
| 关 键 词： | 基体辅助激光解吸电离飞行时间质谱 标记反应 胰岛素 标记蛋白质 生物 素 异硫氰荧光素 |
Study of the Protein Labeling Reactions by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry |
|
| Wei Shoulian,Deng Guanghui,Zheng Yining,Mo Jinyuan. Study of the Protein Labeling Reactions by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2002, 30(2): 162-164 | |
| Authors: | Wei Shoulian Deng Guanghui Zheng Yining Mo Jinyuan |
| Abstract: | In this paper we studied the compositions of commercial biotin and fluorescein isothiocyanate(FITC) labeled insulins, and then in-situ monitored the labeling reaction of insulin by biotin with this method. Some interesting results are obtained:(1) the commercial labeled insulins are the mixtures of different conjugates and unlabeled insulins are always include in them.(2) the molar ratio of label and protein is a main factor to decide the composition of the labeling products, use of the label with high concentration probably leads to the formation of abnormal components. |
| Keywords: | Matrix-assisted laser desorption/ionization-time-of-flight-mass spectrometry insulin labeling reaction biotin fluorescein isothiocyanate |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |