Mixed submerged fermentation with two filamentous fungi for cellulolytic and xylanolytic enzyme production |
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Authors: | Garcia-Kirchner O Muñoz-Aguilar M Pérez-Villalva R Huitrón-Vargas C |
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Institution: | (1) Departamento de Bioprocesos, Unidad Profesional Interdisciplinaria de Biotecnología, Instituto Politécnico Nacional, Av. Acueducto s/n, Col. La Laguna, Ticomán, 07340 México, D. F. México;(2) Departamento de Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, A.P. 70228, 045510 México, D. F. México |
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Abstract: | The efficient saccharification of lignocellulosic materials requires the cooperative actions of different cellulase enzyme
activities: exoglucanase, endoglucanase, β-glucosidase, and xylanase. Previous studies with the fungi strains Aureobasidium sp. CHTE-18, Penicillium sp. CH-TE-001, and Aspergillus terreus CH-TE-013, selected mainly because of their different cellulolytic and xylanolytic activities, have demonstrated the capacity
of culture filtrates of cross-synergistic action in the saccharification of native sugarcane bagasse pith. In an attempt to
improve the enzymatic hydrolysis of different cellulosic materials, we investigated a coculture fermentation with two of these
strains to enhance the production of cellulases and xylanases. The 48-h batch experimental results showed that the mixed culture
of Penicillium sp. CH-TE-001 and A. terreus CH-TE-013 produced culture filtrates with high protein content, cellulase (mainly β-glucosidase), and xylanase activities
compared with the individual culture of each strain. The same culture conditions were used in a simple medium with mineral
salts, corn syrup liquor, and sugarcane bagasse pith as the sole carbon source with moderate shaking at 29°C. Finally, we
compared the effect of the cell-free culture filtrates obtained from the mixed and single fermentations on the saccharification
of different kinds of cellulosic materials. |
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Keywords: | Cellulolytic activity mixture fermentation bagasse pith saccharification |
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