Biased detection of guanine-rich microRNAs by array profiling: Systematic error or biological phenomenon? |
| |
| Institution: | 1. N.N. Petrov Institute of Oncology, St.-Petersburg 197758, Russia;2. St.-Petersburg Pediatric Medical University, St.-Petersburg 194100, Russia;3. Medical Research Institute, Medical and Dental University, Tokyo 113-8510, Japan;4. I.I. Mechnikov North-Western Medical University, St.-Petersburg 191015, Russia;1. Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;2. Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok, Thailand;3. Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;1. Sixth Department of Radiation Oncology, Shandong Cancer Hospital, Jinan, Shandong Province, China;2. School of Medicine and Life Sciences, University of Jinan – Shandong Academy of Medical Sciences, Jinan, Shandong Province, China;3. Department of Radiation Oncology, Cancer Hospital, Tianjin Medical University, Tianjin Province, China;1. Laboratory of Internal Medicine, The First Affiliated Hospital of Wenzhou Medical University, 2 FuXue Road, Wenzhou 325000, China;2. Department of Laboratory Medicine, The Second Affiliated Hospital & Yuying Children''s Hospital of Wenzhou Medical University, 109 Xueyuanxi Road, Wenzhou 325000, China;3. School of Laboratory Medicine, Wenzhou Medical University, Chashan University Town, Wenzhou 325000, China;4. Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, 2 FuXue Road, Wenzhou 325000, China;1. State Key Laboratory of Virology/Institute of Virology, School of Medicine, Wuhan University, Wuhan, China;2. Department of Neurosurgery, Wuhan General Hospital Guangzhou Military Region, Wuhan, China;3. Department of Nephrology, Renmin hospital of Wuhan University, Wuhan, China;4. Department of Clinical Laboratory, Wuhan General Hospital Guangzhou Military Region, Wuhan, China |
| |
| Abstract: | This article describes an unexpected phenomenon which was revealed during the study of microRNA expression profiles of breast tumors. Hierarchical clustering has distinguished two broad groups of microRNAs with different expression patterns. One of these groups, Group Q (“questionable”), was composed mainly of recently discovered microRNAs and contained a large number of viral microRNA species. This microRNA subset was found to be extremely rich in guanine. The above features suggest that the Group Q is an artifact of microRNA expression profiling. However, the latter explanation is not supported by the evidence for biologically relevant associations observed for the Group Q microRNAs. |
| |
| Keywords: | MicroRNA Agilent MicroRNA array Guanine-rich Breast cancer |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
