Determination of flavonoids,phenolic acid and polyalcohol in Butea monosperma and Hedychium coronarium by semi-preparative HPLC Photo Diode Array (PDA) Detector |
| |
| Institution: | 1. Analytical Chemistry Division, Department of Chemistry, DST-FIST Sponsored Department, Mahatma Gandhi Campus, Bhavnagar University, Bhavnagar 364002, India;2. Department of Biochemistry, Junagadh Agricultural University, Junagadh 362001, India |
| |
| Abstract: | The semi preparative HPLC method with PDA Detector was proposed for the determination of one phenolic acid, three flavonoids and one polyalcohol from Butea monosperma and Hedychium coronarium in gradient elution system. The influence of composition of the mobile phase concentration of the mix modifier and temperature on the separation of gallic acid, quercetin, iso-butrin, butrin and eugenol for 90 min was studied. Two different gradient programmes were used to separate these components. The lower limit of quantification of phenolic acid, flavonoids and eugenol is 0.050–0.150 μg/mL and was determined by the least square method and a good correlation was obtained for all separated components. |
| |
| Keywords: | Flavonoid Phenolic acid Polyalcohol HPLC technique Gradient elution |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
