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Micro-plate magnetic chemiluminescence immunoassay and its applications in carcinoembryonic antigen analysis |
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| Authors: | LI ZhiYong ZHANG QianYun ZHAO LiXia LI ZhenJia HU GuoMao LIN Jin-Ming & WANG Shan State Key Laboratory of Environmental Chemistry Ecotoxicology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing China |
| Institution: | LI ZhiYong1,ZHANG QianYun2,ZHAO LiXia1,LI ZhenJia3,HU GuoMao3,LIN Jin-Ming2 & WANG Shan4 1State Key Laboratory of Environmental Chemistry , Ecotoxicology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China,2Department of Chemistry,Tsinghua University,Beijing 100084,3Beijing Chemclin Biotech Co.,Ltd.,Beijing Academy of Science , Technology,Beijing 100094,4School of Chemistry , Chemical Engineering,Xianyang Normal College,Xiany... |
| Abstract: | A micro-plate magnetic chemiluminescence immunoassay was developed for rapid and high throughput detection of carcinoembryonic antigens (CEA) in human sera. This method was based on a sandwich immunoreaction of fluorescein isothiocyanate (FITC)-labeled anti-CEA antibodies, CEA antigens, and horseradish peroxidase (HRP)-conjugated anti-CEA antibodies in mi- cro-plate. The immunomagnetic particles coated with anti-FITC antibodies were used as the solid phase for the immunoassay. The separation procedure was c... |
| Keywords: | micro-plate magnetic chemiluminescence immunoassay carcinoembryonic antigen tumor marker |
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