Interaction of a new cobalt(II) complex of five-coordinated chiral porphyrin with calf thymus DNA |
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Authors: | Tabassum Sartaj Athar Fareeda Arjmand Farukh |
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Affiliation: | (1) Department of Chemistry, Aligarh Muslim University, Aligarh, 202002, India |
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Abstract: | A new template-directed chiral porphyrin [(TPP)Co(Trp)], where TPP = tetraphenylporphyrin and Trp = 1-tryptophan, was prepared and characterized by various physico-chemical methods. Interaction of [(TPP)Co(Trp)] with calf thymus DNA was studied by u.v.–vis. spectroscopy and cyclic voltammetry. The complex [(TPP)Co(Trp)], after interaction with calf thymus DNA, shows a shift in the absorption spectrum and a large hypochromicity, indicating an intercalating binding mode. This observation was further confirmed by the electrochemical behavior of [(TPP)Co(Trp)] before and after interaction with calf thymus DNA. The complex experiences a negative shift in E1/2 and a decrease in Ep. The ratio of cathodic to anodic peak currents ipc/ipa was 1 for [(TPP)Co(Trp)] while for DNA bound complex ipc/ipa 1, suggesting that the calf thymus DNA moiety is bound strongly to the complex [(TPP)Co(Trp)]. Kinetic studies of the DNA-porphyrin complex reveal a psuedo-first order rate law as the plot of kobsversus calf thymus DNA is linear passing through the origin. |
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