Efficient synthesis of a chiral precursor for angiotensin-converting enzyme (ACE) inhibitors in high space-time yield by a new reductase without external cofactors |
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| Authors: | Shen Nai-Dong Ni Yan Ma Hong-Min Wang Li-Juan Li Chun-Xiu Zheng Gao-Wei Zhang Jie Xu Jian-He |
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| Institution: | Laboratory of Biocatalysis and Synthetic Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China. |
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| Abstract: | A new reductase, CgKR2, with the ability to reduce ethyl 2-oxo-4-phenylbutyrate (OPBE) to ethyl (R)-2-hydroxy-4-phenylbutyrate ((R)-HPBE), an important chiral precursor for angiotensin-converting enzyme (ACE) inhibitors, was discovered. For the first time, (R)-HPBE with >99% ee was produced via bioreduction of OPBE at 1 M without external addition of cofactors. The space-time yield (700 g·L(-1)·d(-1)) was 27 times higher than the highest record. |
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