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Fluorescence imaging of potassium ions in living cells using a fluorescent probe based on a thrombin binding aptamer-peptide conjugate
Authors:Ohtsuka Keiichi  Sato Shinobu  Sato Yusuke  Sota Kojiro  Ohzawa Shinsuke  Matsuda Tomoki  Takemoto Kiwamu  Takamune Nobutoki  Juskowiak Bernard  Nagai Takeharu  Takenaka Shigeori
Institution:Department of Applied Chemistry, Graduate School of Engineering, Kyushu Institute of Technology, Fukuoka 804-8550, Japan.
Abstract:When a biotinylated FRET probe based on a peptide-thrombin binding aptamer conjugate was introduced together with streptavidin and biotinylated nuclear export signal peptide into HeLa cells, the resulting ternary complex enabled visualization of K(+) concentration changes in the cell.
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