| Osmotic Regulation of Betaine Content in Leymus chinensis Under Saline-alkali Stress and Cloning and Expression of Betaine Aldehyde Dehydrogenase（BADH） Gene |
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| 作者姓名： | CUI Xi-yan ;WANG Yong ;GUO Ji-xun |
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| 作者单位： | CUI Xi-yan（Key Laboratory for Vegetation Ecology of Ministry of Education,Institute of Grassland Science,Northeast Normal University,Changchun 130024.P.R.China;School of Liye Sciences,Jilin Agricultural University,Changchun 130118,P.R. China） ;
WANG Yong （Key Laboratory for Vegetation Ecology of Ministry of Education,Institute of Grassland Science, Northeast Normal University,Changchun 130024.P.R.China） ;
GUO Ji-xun（Key Laboratory for Vegetation Ecology of Ministry of Education,Institute of Grassland Science, Northeast Normal University,Changchun 130024.P.R.China） ; |
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| 摘 要： | The potted Leymus chinensis seedlings were treated with saline-alkali solution of six different（from Ⅰ to Ⅵ） concentrations. The results demonstrate that the betaine content and Betaine-aldehyde dehydrogenase（BADH： EC 1.2.1.8） activities have a direct relation with increased stressing time in the same treatment; both exhibit a single peak with increasing the concentration of saline-alkali solution, and number V shows the highest value. The BADH gene of Leyrnus chinensis was cloned by RT-PCR and RACE technology and was designated as LcBADH. The cDNA sequence of LcBADH was 1774bp including the open reading frame（ORF） of 1521bp（coding 506 amino acids）. The vector of prokaryotic expression was constructed by inserting the LcBADH gene fragmcnt into pET30a（＋） and transformed into E. coli BL21（DE3）. The result of SDS-PAGE shows that the idio-protein with a molecular mass of 56.78 kDa was effectively expressed in the recombinant bacteria induced by isopropyl fl-D-thiogalactoside（IPTG）.
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| 关 键 词： | 甜菜碱 无性繁殖 脱氢酶 乙醛 活性研究 |
| 收稿时间： | 21 September 2007 |
Osmotic Regulation of Betaine Content in Leymus chinensis Under Saline-alkali Stress and Cloning and Expression of Betaine Aldehyde Dehydrogenase(BADH)Gene |
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| CUI Xi-yan,;WANG Yong,;GUO Ji-xun.Osmotic Regulation of Betaine Content in Leymus chinensis Under Saline-alkali Stress and Cloning and Expression of Betaine Aldehyde Dehydrogenase(BADH)Gene[J].Chemical Research in Chinese University,2008,24(2):204-209. |
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| Authors: | CUI Xi-yan WANG Yong GUO Ji-xun |
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| Institution: | a Key Laboratory for Vegetation Ecology of Ministry of Education, Institute of Grassland Science, Northeast Normal University, Changchun 130024, P. R. China
b School of Life Sciences, Jilin Agricultural University, Changchun 130118, P. R. China |
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| Abstract: | The potted Leymus chinensis seedlings were treated with saline-alkali solution of six different(from Ⅰ to Ⅵ) concentrations.The results demonstrate that the betaine content and Betaine-aldehyde dehydrogenase(BADH:EC 1.2.1.8) activities have a direct relation with increased stressing time in the same treatment;both exhibit a single peak with increasing the concentration of saline-alkali solution,and number V shows the highest value.The BADH gene of Leymus chinensis Was cloned by RT-PCR and RACE technology and Was designated as LcBADH.The cDNA sequence of LcBADH Was 1774bp including the open reading frame(ORF)of 1521bp(coding 506 amino acids).The vector of prokaryotic expression was constructed by inserting the LcBADH gene fragment into pET30a( )and transformed into E. coli BL21(DE3).The result of SDS-PAGE shows that the idio-protein with a molecular mass of 56.78 kDa was effectively expressed in the recombinant bacteria induced by isopropyl β-D-thiogalactoside(IPTG). |
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| Keywords: | Leymus chinensis Saline-alkali stress BADH RACE Cloning and expression |
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