A portable and integrated nucleic acid amplification microfluidic chip for identifying bacteria |
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Authors: | Fang Xueen Chen Hui Xu Lingjia Jiang Xingyu Wu Wenjuan Kong Jilie |
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Affiliation: | Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, PR China. |
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Abstract: | In this work, we developed a portable integrated microchip of loop-mediated isothermal nucleic acid amplification (LAMP). This chip, with sample-to-answer capability, could perform rapid DNA release, exponential signal amplification and naked-eye result read-out in single or multiplex format. We call it iμLAMP, namely integrated micro-LAMP, which was successfully used for point-of-care identification of bacteria. |
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