Cerium-mediated Free Radicals in Contractile Proteins. EPR and DSC study |
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| Authors: | Kiss M. Könczöl F. Farkas N. Lőrinczy D. Belagyi J. |
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| Affiliation: | (1) Central Research Laboratory, Medical School, University of Pécs, Pécs, Hungary;(2) Institute of Forensic Medicine, Medical School, University of Pécs, Pécs, Hungary;(3) Institute of Biophysics, Medical School, University of Pécs, Pécs, Hungary |
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| Abstract: | The effect of free radicals obtained in hydroxyl and cerium(IV)-nitrilotriacetic acid free radical generating systems on contractile proteins (actin, myosin and their complexes in glycerinated muscle fibres) was studied using differential scanning calorimetry and spin trapping electron paramagnetic resonance technique. The analysis of spectra showed that selective attack of thiol groups – Cys-257 and Cys-374 residues of actin, and among others Cys-707 residue of myosin – and random attack of sidechains of the main proteins of muscle tissue produced structural and functional changes, which affected the ATP hydrolysis cycle and very likely the dynamics of actin. The melting curves obtained on protein systems support the view that global conformational changes accompany the local damage of free radicals. This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | contractile proteins DSC hydroxyl and thiyl free radicals spin-trapping EPR |
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