A new high-performance liquid chromatography assay for the determination of sesquiterpene lactone 15-deoxygoyazensolide in rat plasma |
| |
Authors: | Jabor Valquiria Aparecida Polisel dos Santos Michel David Bonato Pierina Sueli Gouvea Dayana Rubio Lopes Norberto Peporine |
| |
Institution: | University of São Paulo, Faculty of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto, SP, Brazil |
| |
Abstract: | A simple, rapid and sensitive high-performance liquid chromatography method was developed for the analysis of the sesquiterpene lactone 15-deoxygoyazensolide (LAC15-D) in rat plasma samples. The chromatographic separation was achieved on a LiChrospher® RP18 column using methanol:water (50:50, v/v) containing 0.6% acetic acid as mobile phase, at a flow rate of 0.7 mL min−1. UV detection was carried out at 270 nm. Phenytoin was used as internal standard. Prior to the analysis, the rat plasma samples were submitted to liquid-liquid extraction with dichloromethane. The mean absolute recoveries were 73% with R.S.D. values lower than 3.5. The method was linear over the 6.0-2000 ng mL−1 concentration range and the quantification limit was 6.0 ng mL−1. Within-day and between-day assay precision and accuracy were studied at three concentration levels (15, 300 and 480 ng mL−1) and were lower than 15%. The validated method was used to measure the plasmatic concentration of LAC15-D in rats that received a single intraperitoneal dose of 30 mg kg−1. |
| |
Keywords: | High-performance liquid chromatography Sesquiterpene lactone 15-Deoxygoyazensolide Antitumor agent Lychnophora ericoides Plasma |
本文献已被 ScienceDirect PubMed 等数据库收录! |