Tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence sensor based on carbon nanotube/organically modified silicate films |
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Authors: | Tao Ying Lin Zhi-Jie Chen Xiao-Mei Chen Xi Wang Xiao-Ru |
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Affiliation: | a Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China b State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen 361005, China |
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Abstract: | In this paper, a novel electrochemiluminescence (ECL) sensor was constructed to determine herring sperm (HS) double-stranded (ds) DNA. Tetramethoxysilane and dimethyldimethoxysilane were selected as co-precursors to form an organically modified silicate (ORMOSIL) film for the immobilization of multiwall carbon nanotubes (MWNTs) wrapped by poly(p-styrenesulfonate) (PSS), and then Tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy)32+) was successfully immobilized on a glassy carbon electrode via ion-association. PSS was employed to increase the conductivity of the ORMOSIL film and disperse the cut MWNTs, which were cut and shortened in a mixture of concentrated sulfuric and nitric acids, in the film. It was found that MWNTs could adsorb Ru(bpy)32+ and acted as conducting pathways to connect Ru(bpy)32+ sites to the electrode. MWNTs also played a key role as materials for the mechanical and thermal properties. The ECL performance of this modified electrode was evaluated in a flow injection analysis (FIA) system, and the detection limit (S/N = 3) for HS ds-DNA was 2.0 × 10−7 g mL−1 with a linear range from 1.34 × 10−6 to 6.67 × 10−4 g mL−1 (R2 = 0.9876). In addition, the ECL sensor presented excellent characteristics in terms of stability, reproducibility and application life. |
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Keywords: | Electrochemiluminescence sensor Carbon nanotubes Tris(2,2&prime -bipyridyl)ruthenium(II) Nucleic acid |
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