Simultaneous determination of 17 ginsenosides in rat urine by ultra performance liquid chromatography-mass spectrometry with solid-phase extraction |
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Authors: | Wang Xiaoyan Zhao Tie Gao Xianfu Dan Mo Zhou Mingmei Jia Wei |
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Institution: | a School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China b Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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Abstract: | A rapid analytical method for quantifying 17 ginsenosides in rat urine by ultra performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) is described. All analytes were extracted by solid-phase extraction optimized to obtain good recovery and quantified using digoxin as an internal standard. ESI-MS was optimized for different cone voltages at positive ionization mode to allow simultaneous analysis of all analytes in a relatively short time. Qualitative methodological considerations, including the linear range, precision, limit of quantification, limit of detection, recovery and sensitivity are also provided. |
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Keywords: | Saponin Ginsenoside Solid-phase extraction Rat urine Ultra performance liquid chromatography-mass spectrometry |
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