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Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of methicillin-resistant Staphylococcus aureus by resonance light scattering |
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| Authors: | Xiao Jian Bo Chen Jing Wen Ren Feng Lian Yang Chun Sheng Xu Ming |
| Affiliation: | a College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China b Yancheng Institute of Technology, Yancheng, Jiangsu 224003, PR China c Research Institute for Molecular Pharmacology and Therapeutics, Central South University, Changsha 410083, PR China d Department of Radiation Oncology, Memorial Sloan-Kettering Cancer Center, NY 10021, USA |
| Abstract: | A rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) using resonance light scattering (RLS) on an ordinary fluorescence spectrometer was developed. The viable MRSA reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to produce insoluble particles which displayed intense resonance scattering light. It showed a linear relationship between the number of viable MRSA and the RLS intensity. Dead MRSA were unable to reduce MTT. MRSA exposed to flavonoids extracted from Marchantia convoluta (MCF) showed a MCF concentration-dependent inhibition of the ability to reduce MTT. The RLS could, in combination with the MTT assay, be a rapid and sensitive detection method for vitro-cultured MRSA. |
| Keywords: | Methicillin-resistant Staphylococcus aureus Resonance light scattering Detection |
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