Influence of the eluent composition on column efficiency in reversed-phase high-performance liquid chromatography |
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| Authors: | J D Wang J Z Li M S Bao P C Lu |
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| Institution: | (1) Dalian Institute of Chemical Physics, Academia Sinica, Dalian, Lianoning, China;(2) Present address: Institut für Organische Chemie, Universität Tübingen, 7400 Tübingen, (German Federal Republic) |
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| Abstract: | Summary The dependence of column efficiency on the eluent (MeOH/H2O) composition in a reversed-phase liquid chromatography system within a wide concentration range has been systematically examined. It is shown that when the intracolumn effect of mass transfer and diffusion is the main factor controlling band broadening the column efficiency decreases with the increase of the viscosity of the MeOH/H2O mixture; on the other hand, when the extra-column effect is the main factor an increase in the viscosity of the eluents will help in improving column efficiency. The column efficiency is also related to the properties of the sample. |
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| Keywords: | Reversed-phase HPLC Small-bore column Column efficiency |
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