| 酪蛋白糖巨肽酶解物的二肽基肽酶-4抑制作用 |
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| 引用本文： | 王 倩,白函瑜,毛学英. 酪蛋白糖巨肽酶解物的二肽基肽酶-4抑制作用[J]. 北京工商大学学报(自然科学版), 2015, 33(2): 19-23 |
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| 作者姓名： | 王 倩 白函瑜 毛学英 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 基金项目： | 国家自然科学基金资助项目，国家级大学生科研创新项目 |
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| 摘 要： | 为探讨酪蛋白糖巨肽(GMP)酶解物的二肽基肽酶-4(DPP-4)抑制作用,选择木瓜蛋白酶、碱性蛋白酶、胰蛋白酶、胃蛋白酶分别酶解GMP获得糖巨肽酶解物,选择甘氨酰脯氨酸对硝基苯胺为底物的发色底物法检测DPP-4活性.结果表明,GMP酶解物的DPP-4抑制效果优于GMP本身,并且随酶解时间的延长酶解物DPP-4抑制作用逐渐增加,其中GMP木瓜蛋白酶解物对DPP-4抑制效果最好,GMP木瓜蛋白酶酶解物作为DPP-4抑制剂具有潜在应用价值.
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| 关 键 词： | 糖巨肽 酶解 DPP-4抑制剂 |
Dipeptidyl Peptidase-4 Inhibitory Activity of Casein Glycomacropeptide Hydrolysates |
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| WANG Qian,BAI Hanyu,MAO Xueying. Dipeptidyl Peptidase-4 Inhibitory Activity of Casein Glycomacropeptide Hydrolysates[J]. Journal of Beijing Technology and Business University:Natural Science Edition, 2015, 33(2): 19-23 |
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| Authors: | WANG Qian BAI Hanyu MAO Xueying |
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| Affiliation: | College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China,College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China and College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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| Abstract: | The aim of the present study was to investigate the dipeptidyl peptidase-4 (DPP-4) inhibitory activity of glycomacropeptide (GMP) hydrolysates obtained with papain, alkaline, trypsin and protease. The DPP-4 activity was assayed by the chromogenic substrate with Gly-Pro-PNA as a substrate in vivo. GMP hydrolysates showed a higher DPP-4 inhibitory rate than native GMP. The DPP-4 inhibitory activity of GMP hydrolysates increased during the hydrolysisperiods. GMP hydrolysates obtained by papain possessed the greatest DPP-4 inhibitory activity. The papain-treated GMP may have the potential application as DPP-4 inhibitor. |
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| Keywords: | glycomacropeptide hydrolysis dipeptidyl peptidase-4 inhibitor |
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