Analysis of 3-chlorotyrosine as a specific marker of protein oxidation: the use of N(O,S)-ethoxycarbonyltrifluoroethyl ester derivatives and gas chromatography/mass spectrometry |
| |
| Authors: | Pietzsch Jens Kopprasch Steffi Bergmann Ralf |
| |
| Affiliation: | Institute of Bioinorganic and Radiopharmaceutical Chemistry, Research Center Rossendorf Dresden, PO Box 51 01 19, 01314 Dresden, Germany. j.pietzsch@fz-rossendorf.de |
| |
| Abstract: | N(O,S)-ethoxycarbonyltrifluoroethyl amino acid esters are formed by the reaction of amino acids with ethylchloroformate plus trifluoroethanol plus pyridine. The use of these derivatives for a rapid and sensitive determination of 3-chlorotyrosine, a highly specific marker of myeloperoxidase-catalyzed protein oxidation, by using standard gas chromatography/electron impact mass spectrometry, is discussed. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
