Fast chiral separations using sulfated beta-cyclodextrin and short-end injection in capillary electrophoresis |
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Authors: | Bergholdt A B Jørgensen K W Wendel L Lehmann S V |
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Affiliation: | Analytical Development, Pharmaceuticals Development, Novo Nordisk A/S, Maaloev, Denmark. |
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Abstract: | The general applicability of sulfated beta-cyclodextrin as chiral selector and short-end injection in capillary electrophoresis (CE) as a powerful screening tool for fast and efficient chiral separation of Ormeloxifene enantiomers and racemic Ormeloxifene analogues is demonstrated. Using the short-end injection procedure, all of the 16 racemic compounds studied were successfully separated with high efficiencies and with analysis times of less than 1.2 min. Furthermore, long-end injections of eight analogues named C1-C8 afforded separations with extremely high efficiencies. A statistical evaluation of the resolution values obtained in short-end and long-end injections of compounds C1-C8 showed that the sensitivity of the CE method towards structural changes in the studied molecules is intact when the chiral analysis is performed with short-end injection compared to conventional long-end injection. |
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