六味地黄丸三维同步荧光光谱特性的研究

应用英国Edinburgh公司FLS920P荧光光谱仪，实验测定了六味地黄丸组分中熟地黄、山茱萸、山药、泽泻、茯苓、牡丹皮等六味中药的三维同步荧光光谱，并提取荧光特征参数，发现六味中药均为荧光物质，谱图线形各不相同，具有指纹特性，且与药理相吻合。进一步，实验测定六味地黄丸的标准水煎剂及非标准水煎剂的三维同步荧光光谱，发现两者差异明显，可用于区分不同组方的中成药水煎剂。实验与理论均表明：将三维荧光光谱分析方法与同步荧光光谱分析方法相结合，可以进一步提高荧光光谱技术的灵敏度和选择性，在多组分复杂混合物的荧光光谱分析中有明显的优势。因此，本文的工作为建立完整信息中药材指纹图谱提供了一种方便快捷可靠的方法，也可为中成药的品质鉴别和成分分析提供帮助。

The Study of Three-Dimensional Synchronous Fluorescence Spectral Characteristics of Liuwei Dihuang Pills

Three-dimensional synchronous fluorescence of each component of Liuwei dihuang pills (Prepared rehmannia root, cornel,yam,alisma,poria cocos,and cortex moutan)is measured by using FLS920P fluorescence spectrometer.Feature param-eters were extracted.It can be found that each component is fluorescent material and the lines are all different.Furthermore, Three-dimensional synchronous fluorescence of Liuwei dihuang pills boiling with standard water and non-standard water are all measured and there are significant differences between them.It can be applied in distinguishing different formula of Chinese med-icine decoction.Experimental and Theoretical conclusion show that:the three-dimensional fluorescence spectrometry method and the combination of synchronous fluorescence spectroscopy method can further improve the sensitivity and selectivity of fluores-cence spectroscopy,have a distinct advantage in a complex multi-component mixture of fluorescence spectroscopic analysis.The study can provide a convenient and reliable method for establishing a complete fingerprint of Chinese traditional medicine.It also can help identifying the component and the quality of Chinese patent medicine.