Preparation of Pt/polypyrrole-ferrocene hydrogen peroxide sensitive electrode for the use as a biosensor

A polypyrrole electrode with ferrocene mediator is prepared and its sensitivity to hydrogen peroxide is investigated. The polypyrrole is deposited upon a 0.5 cm² Pt plate by the polymerization of pyrrole by scanning the electrode potential between 0.0 and 0.9 V at a scan rate of 50 mV/s. The platinum/polypyrrole-ferrocene (Pt/PPy-Fc) electrode is prepared by adding ferrocene to the coverage medium. The electrode’s sensitivity to hydrogen peroxide is investigated at room temperature using 0.025 M phosphate buffer at pH 7. The working potential is 0.7 V, the concentrations of pyrrole and ferrocene are 0.2 M and 10 mM. Polypyrrole was coated on the electrode surface within 26 cycles. Published in Russian in Elektrokhimiya, 2006, Vol. 42, No. 2, pp. 160–164. The text was submitted by the authors in English.