Adsorption of lysozyme on a hemodialysis sulfonated polyacrylonitrile membrane, with and without preadsorbed poly(ethyleneimine) on the external faces |
| |
Authors: | Jocelyne Ethve Philippe Djardin Michel Boissire |
| |
Institution: | Laboratoire Materiaux Procedes Membranaires, Université Montpellier II, European Membrane Institute, UMR 5635 (ENSCM-UMII-CNRS), 2 Place Eugene Bataillon, CC 047, 34095, Montpellier, Cedex 5, France |
| |
Abstract: | We present the influence of pH, from pH 4 to 10, with a focus on the neutral range, on the adsorption of lysozyme (isoelectric point pI=11) on a sulphonated membrane and the same membrane pre-treated with poly(ethyleneimine) (PEI). We found a steep increase of the adsorbed amount above pH 6 in phosphate buffer. The adsorbed amount was about twice as low in Tris buffer, around the neutral pH. The difference between the two types of buffer is attributed to their different ionic composition. High interfacial concentration in phosphate buffer is especially linked to the phosphate divalent anions. In the presence of divalent sulphate anions, we measured the same level of interfacial concentration than with phosphate buffer. With the PEI pre-treated membrane, we observed, on the time scale of our experiments (15–20 h), similar adsorbed amounts than on the raw membrane, showing that the PEI layer does not constitute a true barrier to the penetration of lysozyme into the membrane core. However, its presence leads to a slower adsorption rate in a system where convection does not occur through the membrane. |
| |
Keywords: | Lysozyme Adsorption Hemodialysis Poly(ethyleneimine) Protein |
本文献已被 ScienceDirect 等数据库收录! |
|