两种有机磺酸配合物的合成、表征及与DNA键合性质

合成了2个新型有机磺酸配合物，[Cd(phen)₂(ans)₂]·H₂O (1)和[Pb(phen)₂(ans)₂]·H₂O (2) (phen=1，10-邻菲咯啉，ans=4-氨基-1-萘磺酸根)，通过元素分析、红外光谱等对配合物进了表征，用X-射线单晶衍射方法测定了配合物的单晶结构。应用紫外-可见吸收光谱、荧光光谱及粘度测定方法研究了配合物与ctDNA的作用，发现2个配合物均以插入和氢键两种模式与ctDNA发生作用。

Syntheses, Characterization, Crystal Structures and DNA-binding Properties of Two Complexes Containing Organosulfonate Ligand

Two organosulfonate complexes, [M(phen)2(ans)2]·H2O (M=Cd 1, M=Pb 2), were obtained from the reaction of 1,10-phenanthroline (phen), sodium 4-aminonaphthalene-1-sulfonate tetrahydrate (Naans) and Cd(Oac)2·2H2O or Pb (NO3)2 in a mixed solvent of water and methanol at 25℃. The compounds were characterized by elemental analysis, IR, and X-ray diffraction single crystal structure analyses. Interactions of the complexes with calf thymus DNA (ctDNA) were investigated by UV-Vis spectra, luminescence spectra (including luminescence titrations, steady-state emission quenching by [Fe(CN)6]4-, DNA competitive binding with ethidium bromide (EB)), and viscosity measurements. The experimental results indicated that there were two interactions between the complexes and DNA, namely the electrostatic interaction and intercalation, with the binding constants of 1.82×105L·mol-1 for 1 and 4.96×104L·mol-1for 2 in buffer of 50 mmol·L-1 NaCl and 5 mmol·L-1 Tris-HCl (pH 7.0). CCDC: 669692, 1; 674923, 2.