Enzymatic determination of citrulline in biological samples |
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| Authors: | Wolfgang Eichler |
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| Affiliation: | (1) Institut für Biochemie I der Universität Heidelberg (Med. Fak.), Im Neuenheimer Feld 328, D-6900 Heidelberg, Federal Republic of Germany |
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| Abstract: | Conclusions The newly developed method for the enzymatic determination of citrulline in biological specimens gives satisfying and reproducible results and is apt for measuring citrulline concentrations in the physiological as well as in the pathological range, for disease screening and probably can be utilized for automatic analysis.Since citrulline hydrolase is a fairly stable enzyme with no requirements for cofactors and not interfered by basic amino acids and urea, this method offers a true alternative to the citrulline-determinations employed previously.
Enzymatische Bestimmung von Citrullin in biologischen Proben |
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