Low cost optical detection of DNA hybridization on biochips |
| |
Authors: | Ch. Pieralli Ch. Attoui W. Boireau B. Wacogne |
| |
Affiliation: | 1. Optics Department, FEMTO-ST Institute, UFR ST, 16, route de Gray, F-25030, Besan?on cedex, France 2. CLinic-Innovation Proteomic Platform, FEMTO-ST Institute, 32, avenue de l’Observatoire, F-25044, Besan?on cedex, France
|
| |
Abstract: | The detection of DNA hybridization can be either complicated or require expensive devices. Some methods use a fluorescence signal to investigate hybridization. Here, we present an optical probe based on optical fibers both for illumination and for fluorescence collection. The detection is made with a microspectrophotometer and the signal is then treated with a PC. We also developed the DNA biochips (glass plate with a gold layer functionalized with target DNA) as well as a dedicated microtank which maintains a constant flow of probe DNA over the target one. Finally, the detection of hybridization with this simple and versatile system is presented. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|