Cellular and molecular responses of E. fetida c?lomocytes exposed to TiO2 nanoparticles |
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Authors: | Emilie Bigorgne Laurent Foucaud Céline Caillet Laure Giambérini Johanne Nahmani Fabien Thomas Fran?ois Rodius |
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Institution: | 1. Universit?? de Lorraine??Laboratoire des Interactions Ecotoxicologique Biodiversit?? Ecosyst??mes (LIEBE), CNRS UMR7146, Rue du g??n??ral Delestraint, 57070, Metz, France 2. Universit?? de Lorraine??Laboratoire Environnement et Min??ralurgie (LEM) CNRS UMR7569, 15, Avenue du Charmois, BP 40, 54501, Vandoeuvre Cedex, France
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Abstract: | An in vitro approach using c?lomocytes of Eisenia fetida was investigated to evaluate toxicity of TiO2 nanoparticles. C?lomocytes were exposed to well-dispersed suspension of small aggregates (130?nm) of TiO2 nanoparticles (1?C25???g/ml) during 4, 12 and 24?h. Intracellular localisation suggested that the main route of uptake was endocytosis. Cellular responses showed that TiO2 nanoparticles were not cytotoxic and had no effect on phagocytosis at any of the four concentrations for each time tested. Concerning molecular responses, an increase of fetidin and metallothionein mRNA expression was observed starting from 4?h of exposure. In contrast, expression of coelomic cytolytic factor mRNA decreased for 10 and 25???g/ml after 4?h. Superoxide dismutase, catalase and glutathione-S-transferase expression were not modified suggesting that oxidative stress was not induced by TiO2 in our experimental conditions. This in vitro approach showed that TiO2 nanoparticles were taken up by c?lomocytes and they could modify the molecular response of immune and detoxification system. |
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