石墨烯-壳聚糖-纳米金修饰的免疫传感器应用于1-芘丁酸的检测研究

本文采用石墨烯(GS)-壳聚糖(CS)-纳米金(Nano-Au)复合材料修饰玻碳电极,构建性能良好的生物识别界面,制备一种无标记的电流型免疫传感器,并应用于1-芘丁酸(PBA)的高灵敏检测。研究结果表明,GS、CS和Nano-Au的协同作用,极大地提高了anti-PAHs抗体在电极表面的覆盖量,从而提高了免疫传感器的灵敏度和检测性能。采用示差脉冲伏安法(DPV)对PBA进行检测,PBA在0.001~10ng/mL和10~200ng/mL浓度范围内与峰电流值呈良好的线性关系,检出限为0.001ng/mL。该传感器应用于实际水样中痕量PBA的检测,加标回收率为90%~105%。

Study on Graphene-Chitosan-Nano-Au Modified Amperometric Immunosensor for the Determination of 1-Pyrenebutyric Acid

A simple,highly sensitive,and label-free electrochemical amperometric immunosensor was developed based on graphene (GS),chitosan (CS) and Nano-Au composites modified glassy carbon electrode for the determination of 1-pyrenebutyric acid (PBA).The morphology and electrochemical properties of the immunosensors were characterized by transmission electron microscopy and cyclic voltammetry in detail.The study displayed that the amount of immobilization antibody-PAHs was remarkably increased due to the synergistic effect of GS,CS and Nano-Au,resulting in that the sensitivity and detective performance of the proposed immunosensors were significantly enhanced.The amperometric response was proportional to the PBA in the range from 0.001 to 10 and from 10 to 200 ng/mL with a detection limit of 0.001 ng/mL.The proposed immunosensor was applied to detect trace PBA in real water samples,and the recoveries for spiking experiments were 90％-105 ％ with satisfactory results.