高选择性半胱氨酸荧光探针的合成及生物成像

生物巯基分子在人体生理和病理过程中发挥着重要作用。因此,专一性识别生物巯基分子在生命科学领域具有非常重要的应用价值。我们利用醛基作为巯基识别基团进行成环反应,合成了一种新型的荧光增强型半胱氨酸(Cys)荧光探针,其结构用核磁共振氢谱、质谱以及红外光谱进行表征。该探针分子具有合成简单、选择性好、光稳定性好等优点,本身无色,无荧光,与Cys作用后,紫外吸收光谱蓝移约15nm,荧光强度增强105倍,荧光颜色由无色变为深蓝色,可实现裸眼观测且能进行活细胞成像。

Synthesis and Biological Imaging of Highly Selective Fluorescent Probe for Cysteine

Biothiols play an important role in the physiological and pathological processes.Therefore,the specific discrimination of biothiols in biological samples is of great importance in life sciences.In this work,a novel fluorescene-enhanced probe for cysteine was synthesized successfully and then characterized by means of 1H NMR,MS and IR.The probe showed many advantages including easy synthesis,good selectivity and high photostability.The free probe was colorless and non-fluorescent.An apparent color change from colorless to deep blue was observed upon addition of Cys with a～ 15 nm blue-shift in absorption spectra and 105-fold fluorescence intensity enhancement,which can serve as a "naked-eye" probe for Cys and achieve good imaging in living cells.