| 光度法快速分析苯乙酮酸含量的研究 |
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| 引用本文： | 李桂银,黄可龙,蒋玉仁,丁萍.光度法快速分析苯乙酮酸含量的研究[J].分析科学学报,2007,23(6):734-736. |
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| 作者姓名： | 李桂银 黄可龙 蒋玉仁 丁萍 |
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| 作者单位： | 1. 中南大学化学化工学院,长沙,410083;湖南科技职业学院轻化工程学院,长沙,410118
2. 中南大学化学化工学院,长沙,410083 |
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| 基金项目： | 国家自然科学基金(No.20376085) |
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| 摘 要： | 研究了一种快速定量分析发酵液中苯乙酮酸的方法。在氢氧化钠溶液中,苯乙酮酸和2,4-二硝基苯肼形成一种红棕色的溶液,该溶液在458 nm处有最大吸收,据此可以快速分析混合物中苯乙酮酸的含量。该法在微生物还原苯乙酮酸生产D-扁桃酸的工艺中进行样品分析,结果表明方法简便、快速、灵敏度高,可以用于监控反应进程。
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| 关 键 词： | 苯乙酮酸 D-扁桃酸 分光光度法分析 |
| 文章编号： | 1006-6144（2007）06-0734-03 |
| 收稿时间： | 2006-10-08 |
| 修稿时间： | 2006-11-22 |
Analysis of the Content of Benzoylformic Acid in Mixture by Colorimetry |
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| LI Gui-yin,HUANG Ke-long,JIANG Yu-ren,DING Ping.Analysis of the Content of Benzoylformic Acid in Mixture by Colorimetry[J].Journal of Analytical Science,2007,23(6):734-736. |
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| Authors: | LI Gui-yin HUANG Ke-long JIANG Yu-ren DING Ping |
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| Abstract: | It is found that the content of benzoylformic acid in ferments can be analyzed quickly and conveniently by colorimetry.In the aqueous solution of sodium hydroxide,benzoylformic acid can coordinate with 2,4-dinitrophenylhydrazine to form a complex which has maximum absorption at 458 nm.Thus the content of benzoylformic acid can be quantitatively determined.Applied to the determination of sample solution in the process of D-mandelic acid production by microorganism bioreduction,the proposed method is proven to be simple,rapid and sensitive,and can be used as an effective means to monitor the process of D-mandelic acid production. |
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| Keywords: | Benzoylformic acid D-mandelic acid Colorimetry |
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