Determination of Penicillium roqueforti toxin by reversed-phase high-performance liquid chromatography. |
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| Authors: | K Siemens J Zawistowski |
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| Institution: | Manitoba Research Council, University of Manitoba, Food Science Department, Winnipeg, Canada. |
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| Abstract: | A method for the detection and quantification of Penicillium roqueforti toxin (PRT) using reversed-phase high-performance liquid chromatography has been established. The limit of quantitation of this method was 3 ng of PRT, while the limit of detection was 2 ng of toxin. The precision of the analysis based on numerous runs was good. Retention times for PRT were highly reproducible with an average coefficient of variation of about 1.6%. Analysis of PRT in liquid and solid samples showed no interference of the sample matrix. The accuracy of the method was 98.6%, with mean PRT recoveries of 96.8%, and 100.4% for the spiked culture medium and blue cheese extracts, respectively. |
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