毛细管气相色谱法测定眼科手术粘弹剂中乳酸含量

采用毛细管气相色谱法测定眼科手术粘弹剂中乳酸的含量。乳酸与甲醇酯化反应后,选用氯仿提取其中酯类物质,进行GC分析。采用DB–Wax毛细管柱(30 mm×0.32 mm,0.5μm),FID检测器,进样口温度为250℃,检测器温度为250℃。采用程序升温,起始温度为70℃,以5℃/min升温至100℃,再以25℃/min升至220℃,保持2 min。在选择的色谱条件下,酯化后的乳酸得到良好的分离,乳酸线性范围为0.089 4～1.165 2 mg/mL(r=0.999 6),检出限为0.15μg/mL。方法的加标回收率为97.8%～98.8%,测定结果的相对标准偏差为1.97%(n=6)。该方法样品前处理简单,干扰少,重现性好,分离效率高,可作为眼科手术粘弹剂中功能成分乳酸质量控制的方法。

Determination of Lactic acid in Ophthalmologic Surgical Viscoelastic Agents by Capillaty Gas Chromatography

The method for the determination of lactic acid in ophthalmologic surgical viscoelastic agent by capillaty gas chromatography was established. After the esterication of lactic acid with methanol, chloroform was used to extract the esterication product for GC analysis. ADB-Wax column （30 mm×0.32 mm, 0.5 μm） and FID were used. The injector and detector temperature was 250℃. Column temperature was controlled by the program：initial temperature of 70℃was raised to 100℃at 5℃/min, then raised to 220℃（2 min） at 25℃/min. Under the optimised conditions, esterification of lactic acid was well separated. Standard addition test results showed that the recoveries were 97.8%-98.8%, the relative standard deviation was 1.97%（n=6）. The detection limit was 0.15 μg/mL. This method is simple,fast,sensitive and accurate,and it can be used for mass control of lactic acid content in ophthalmologic surgical viscoelastic agents.