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Radiotracer studies of the antitumor metallocene molybdocene dichloride with biomolecules |
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| Authors: | Kate S Campbell Carolyn T Dillon Suzanne V Smith Margaret M Harding |
| Institution: | 1. School of Chemistry, The University of Sydney, NSW 2006, Australia;2. Department of Chemistry, University of Wollongong, NSW 2522, Australia;3. Institute of Materials and Engineering Science, Australian Nuclear Science and Technology Organization, Private Mail Bag 1, Menai, NSW 2234, Australia;4. School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia |
| Abstract: | Neutron irradiation of Cp2MoCl2 for 24 h afforded the radiotracer Cp299MoCl2 which was characterised by UV–Vis spectroscopy and thin layer chromatography. Binding experiments with the thiol containing protein human serum albumin (HSA) or calf thymus DNA, were monitored for 99Mo using a gamma counter. Under the conditions investigated, molar ratios of binding of 0.2:1 (Cp2MoCl2:DNA) and 9.4:1 (Cp2MoCl2:HSA) were calculated. The results are consistent with in vitro coordination studies that have shown strong preferential interaction of Cp2MoCl2 with thiols versus other donor sites in biomolecules including DNA. |
| Keywords: | Molybdocene dichloride Antitumor metallocene Genotoxicity 99Mo-radiotracer DNA Human serum albumin |
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