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黄酮化合物的偶氮显色反应及其应用研究
引用本文:余倩,陈小康,罗宗铭,杨迟,麦桦坤.黄酮化合物的偶氮显色反应及其应用研究[J].光谱学与光谱分析,2004,24(8):991-994.
作者姓名:余倩  陈小康  罗宗铭  杨迟  麦桦坤
作者单位:广东工业大学轻工化工学院,广东,广州,510090;韶关学院化学系,广东,韶关,512005
基金项目:国家自然科学基金 (2 0 1 0 50 0 3),广东省自然科学基金(980 1 2 8)资助项目
摘    要:在pH 10 4的NH4Cl NH3 ·H2 O缓冲溶液中 ,对磺基氯化重氮苯可与芦丁 ,桑色素 ,染料木甙 ,槲皮素 ,橙皮甙 ,黄岑甙和大豆甙等 7种黄酮化合物发生偶氮显色反应 ,反应产物的最大吸收波长 ,除黄岑甙为 380nm外 ,其余均在 4 30nm ,显色反应的灵敏度依上述顺序逐渐减弱。其中芦丁的摩尔吸收光系数最大 ,为 3 2 8× 10 4L·mol-1·cm-1,大豆甙摩尔吸光系数最小 ,为 8 30× 10 2 L·mol-1·cm-1。提出的分光光度分析方法 ,用于槐米和豆粕中黄酮化合物的测定 ,RSD为 0 2 5 %~ 4 4 8% ,回收率在 99 3%~ 10 5 0 %之间

关 键 词:黄酮化合物  偶氮显色反应  分光光度法  槐米  豆粕
文章编号:1000-0593(2004)08-0991-04
修稿时间:2003年12月16

Azoic Color Reaction of Flavonoids and Their Analytical Application
YU Qian ,CHEN Xiao kang ,LUO Zong ming ,YANG Chi ,MAI Hua kun . Faculty of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou ,China.Azoic Color Reaction of Flavonoids and Their Analytical Application[J].Spectroscopy and Spectral Analysis,2004,24(8):991-994.
Authors:YU Qian  CHEN Xiao kang  LUO Zong ming  YANG Chi  MAI Hua kun Faculty of Chemical Engineering and Light Industry  Guangdong University of Technology  Guangzhou  China
Institution:Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510090, China.
Abstract:In NH4Cl-NH3 H2O buffer solution of pH 10.4, diazo salt of p-sulfodiazoben-zene chloride reacts with rutin, morin, genistin, quercetin, hesperidin, baicalin and daidzin to form color azo-compounds. These azo-compounds have an absorption maximum at 430 nm except for baicalin azo-compound, which has an absorption maximum at 380 nm. The sensitivity of azoic color reaction is reductive in the order of above flavonoids and the molar absorptivity is maximum for rutin and is minimal for daidzin among these azo-compounds, i. e. 3.28 x 10(4) and 8.3 x 10(2) L x mol(-1) x cm(-1) respectively. The proposed spectrophotometry has been applied to th determination of flavonoids in sophora seed and soybean cake. The RSDs of the method were found to be in the range of 0.75-4.48%, and the recoveries were in the range of 99.3%-105.0%.
Keywords:Flavonoid  Azoic color reaction  Spectrophotometry  Sophora seed  Soybean cake
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