| 二锌胰岛素质子二维核磁共振谱的研究——甲基,亚甲基共振峰的确定 |
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| 引用本文： | 吴家振. 二锌胰岛素质子二维核磁共振谱的研究——甲基,亚甲基共振峰的确定[J]. 波谱学杂志, 1988, 5(3): 265-271 |
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| 作者姓名： | 吴家振 |
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| 作者单位： | 中国科学院生物物理所 |
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| 摘 要： | 用二维核磁共振的方法确定二锌胰岛素质子核磁共振谱中的甲基,亚甲基基团共振峰。结合二锌胰岛素x-射线晶体结构所提供的信息,通过欧弗豪斯效应二维偶极相关谱(NOEsY)已确定10个异亮氨酸,亮氨酸,缬氨酸,苏氨酸甲基基团共振峰。由偶合相关二维谱(COSY)交叉峰的分析可确定13个其它基团的共振峰的归属。在pH=11.0时,二锌胰岛素以单体形式出现。自旋扩散不是一个严重问题。
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| 关 键 词： | 二维核磁共振谱 胰岛素 共振峰的确定 |
| 收稿时间： | 1987-07-13 |
PROTON NMR STUDIES OF 2-Zn INSULIN ASSIGNMENT OF METHYL AND METHYLENE GROUP RESONANCES FROM TWO-DIMENSIONAL SPECTRA |
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| Wu Jiazhen. PROTON NMR STUDIES OF 2-Zn INSULIN ASSIGNMENT OF METHYL AND METHYLENE GROUP RESONANCES FROM TWO-DIMENSIONAL SPECTRA[J]. Chinese Journal of Magnetic Resonance, 1988, 5(3): 265-271 |
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| Authors: | Wu Jiazhen |
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| Affiliation: | Institute of Biophysics, Academia Sinica, Beijing China |
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| Abstract: | Two - Dimensional NMR methods were used to assign methyl and methylene group resonances in the NMR spectra of 2-Zn insulin. 10 methyl group of Ile, Leu, Val,Thr resonances are specifically assigned using nuclear overhauser effect ( NOESY ) spectra of insulin in combination with the information of X-ray structure.Assignment of other group resonances were based on the analysis of Two-Dimensional spectra of the J-Correlated ( COSY ) .Insulin exists in the monomeric state at pH 11.0, where spin diffusion is not a serious problem. |
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| Keywords: | 2-D NMR spectra Insulin Assignment of resonances. |
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