One-pot ligation strategy for atypical ubiquitin chains synthesis by using the trifluoroacetamidomethyl-protected isopeptide-linked Ub (Tfacm-isoUb) unit |
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| Authors: | Yan-Yan Si Lu-Jun Liang Shan Tang Yun-Kun Qi Yong Huang Ji-Shen Zheng |
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| Institution: | 1. Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University, Shenzhen Graduate School, Shenzhen 518055, China;2. School of Life Sciences, University of Science and Technology of China, Hefei 230027, China |
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| Abstract: | Protein chemical synthesis can provide the homogeneous atypical ubiquitin chains that are usually difficult to obtain by other methods. Herein, we report a new one-pot ligation approach for the synthesis of atypical Ub chains based on the Tfacm-protected isoUb building block with operational simplicity and high efficiency. The key intermediate, the Tfacm-protected isoUb building block can be readily prepared by Fmoc SPPS. The practicality and efficiency of the new method is demonstrated by the successful preparation of K11-linked di-Ub. |
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| Keywords: | Atypical ubiquitin chains One-pot native chemical ligation Isopeptide-linked Ub (isoUb) Trifluoroacetamidomethyl group |
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