Smart optical probe for ‘equipment-free’ detection of oxalate in biological fluids and plant-derived food items |
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Authors: | Nilanjan Dey Namita Kumari Deepa Bhagat Santanu Bhattacharya |
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Institution: | 1. Indian Institute of Science, Bangalore, 560012, Karnataka, India;2. Indian Council of Agricultural Research, National Bureau of Agriculturally Insect Resources, Bangalore, 560 024, India;3. Director''s Research Unit, Indian Association of Cultivation of Science, Kolkata, 700032, India |
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Abstract: | A reversible ‘turn-on’ sensor has been designed for ‘naked-eye’ detection of oxalate at nanomolar concentration (~12.5?nM) at pH 7.4. The sensory system shows a highly specific response towards oxalate among a wide range of antinutrients and biologically relevant anionic species. Mechanistic investigations indicate that oxalate can turn the pink-colored solution colorless by dissociating the preformed metal complex. Additionally, high specificity and good accuracy with recovery values ranging from 93.3 to 105.0% were obtained during oxalate estimation in spiked water and human urine samples, confirming the suitability of the present method in estimating trace-level of oxalate in complex matrices. With these results, quantitative estimations of endogenous oxalate were achieved in more than twenty-five different agricultural crops. Finally, low-cost, portable paper strips were developed for on-site detection of oxalate. |
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Keywords: | Oxalate Naked-eye sensing Urine samples Oxalate rich vegetables Displacement approach |
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