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An ultrasensitive rapid-response fluorescent probe for highly selective detection of HSA |
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| Authors: | Pengbo Li Yiru Wang Shufang Zhang Liang Xu Gongchen Wang Jingnan Cui |
| Institution: | 1. Dalian Sixth People Hospital, Dalian 116031, China;2. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China;3. Dalian Medical University, Dalian 116044, China;4. School of Chemistry, Dalian University of Technology, Dalian 116024, China |
| Abstract: | A fluorescent probe HCAB based on twisted intramolecular charge transfer (TICT) mechanism has been designed and synthesized by introducing benzoyl moiety to 4-dimethylamino-2′-hydroxychalcone. HCAB showed excellent selectivity towards human serum albumin (HSA) among different proteins with a remarkable 160-fold fluorescence enhancement and a wider linear range 0–100?mg/L of HSA. Job’s Plot analysis suggested that the formation of HCAB-HSA complex followed a 1:1 stoichiometry. Molecular docking and the displacement assay demonstrated the binding site of HCAB was subdomain IIA and IB of HSA. We also tested HSA levels in human plasma for practical application, the results obtained from HCAB method were similar to those obtained from the standard clinical method. |
| Keywords: | Human serum albumin Fluorescent probe 2′-Hydroxychalcone TICT Rapid detection |
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