Affinity binding on argon plasma-immobilized biotinylated phospholipids |
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Authors: | Mark E. Blaylock Allan S. Hoffman |
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Affiliation: | (1) Center for Bioengineering, University of Washington, 98195-1750 Seattle, Washington |
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Abstract: | Dioleoylphosphatidylcholine (DOPC) and N-biotinyl-dioleoylphosphatidylethanolamine (bDOPE) were covalently bonded to polystyrene surfaces by exposure to argon radio frequency (RF) plasmas. Characterization by electron spectroscopy for chemical analysis (ESCA) for P-, N- and O-contents revealed net increases in oxygen species, but no significant changes in P/N ratios due to the different plasma treatments. Plasma-immobilized bDOPE was shown to bind avidin by affinity interactions. Nonspecific adsorption of avidin was comparable on plasma-immobilized DOPC and bDOPE-DOPC mixed-lipid coatings. |
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Keywords: | Phospholipids dioleoylphosphatidylcholine plasma-immobilized biotinylated-DOPE |
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