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形体细胞的MCEM模型及其光散射分布特征
引用本文:王亚伟,崔青义,卜敏,洪云,刘莹,吴大建.形体细胞的MCEM模型及其光散射分布特征[J].应用光学,2007,28(1):115-120.
作者姓名:王亚伟  崔青义  卜敏  洪云  刘莹  吴大建
作者单位:江苏大学,理学院,江苏,镇江,212013
基金项目:江苏大学校科研和教改项目
摘    要:针对光散射细胞微粒测量中真实形体细胞的取向对测量结果的影响问题,基于Rayleigh-Debye-Gans理论和双椭球核式(CEM)模型在应用中的差异,建立了CEM的修正模型(MCEM),根据此模型系统讨论了细胞在不同入射角变化情况下其光散射强度分布所产生的变化,得到了不同入射角下有核细胞光散射强度分布与有核细胞相关物理特征量的动态响应关系;此外,对有核细胞光散射强度分布进行拟合,得到了有核细胞光散射强度分布函数。误差分析表明:拟合结果可有效地应用于真实细胞光散射测量中的数值反演计算。

关 键 词:光学测量  细胞微粒  光散射  粒度分布
文章编号:1002-2082(2007)01-0115-06
收稿时间:2006/6/23
修稿时间:2006-06-232006-10-10

Light-scattering distribution and MCEM model of shape-cells
WANG Ya-wei,CUI Qing-yi,BU Min,HONG Yun,LIU Ying,WU Da-jian.Light-scattering distribution and MCEM model of shape-cells[J].Journal of Applied Optics,2007,28(1):115-120.
Authors:WANG Ya-wei  CUI Qing-yi  BU Min  HONG Yun  LIU Ying  WU Da-jian
Institution:The Faculty of Science, Jiangsu University, Zhenjiang 212013, China
Abstract:Based on the application difference in Rayleigh-Debye-Gans theory and CEM model,MCEM(modify co-central ellipse model) is established to eliminate the influence of the orientation effect of the shapecell particles on measurement result in light scattering cell particles.Based on the MCEM theory,the variation of the light-scattering intensity distribution of cells is discussed systematically at the different incidence angles,the dynamic response relationship between the light-scattering intensity distribution and the relevant physical characteristic of the karyocyte at the different incidence angles was obtained.In addition,the scattering intensity distribution function of the karyocyte is acquired by fitting the scattering intensity distribution.The error analysis shows that the fitting result can be used in the numerical inversion calculation effectively for the light-scattering measurement of the real cell.
Keywords:optical measurement  cell particle  light-scattering  granularity distribution
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