Improved deprotection in solid phase peptide synthesis: quantitative reduction of methionine sulfoxide to methionine during HF cleavage |
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Authors: | James P Tam William F Heath RB Merrifield |
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Institution: | The Rockefeller University, 1230 York Avenue, New York 10021 U.S.A. |
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Abstract: | The reduction of methionine sulfoxide residues in peptides was found to be quantitative by treatment with a low concentration of HF in dimethylsulfide (HF:dimethylsulfide, 1:3, v/v). The reduction was dependent on the acidity function of HF and was optimal with an HF concentration between 25% and 40%. The low HF concentration cleavage reagent also deprotected most of the benzyl alcohol-derived protecting groups. |
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