聚甲基丙烯酸-聚乙烯吡啶复合物固定纤维素酶的研究

<正> 作为植物细胞壁主要成份的纤维素,占植物总重的一半,是地球上最丰富的有机物,是对人类有用而且潜力很大的自然资源。将这些廉价易得的纤维素利用纤维素酶转变成葡萄糖,不仅在食品工业上有现实意义,而且对未来化学工业的发展有着不可估量的影响。但由于酶在水溶液中不稳定,反应后难以分离和回收,只能使用一次,成本较高,使其应用受到一定的限制,固定酶的发展正好弥补了上述不足。

STUDIES ON IMMOBILIZATION OF CELLULASE BY THE COMPLEX OF POLYMETHACRYLIC ACID AND POLY-4- VINYLPYRIDINE

An immobilized cellulase was prepared by the immobilization of cellulase with a complex of polymethacrylic acid and polyvinylpyridine for the use of transformation of cellulose to glucose. It has been found that the yield of glucose is affected by molecular weight of polymer support, pH value of solution, reaction temperature and so on. The immobilized cellulase was more stable than the soluble cellulase. It could be reused ten times and stored for 25 days without appreciable loss in catalytic activity.