Ultra high performance liquid chromatography with ion‐trap TOF‐MS for the fast characterization of flavonoids in Citrus bergamia juice |
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| Authors: | Eduardo Sommella Giacomo Pepe Francesco Pagano Gian Carlo Tenore Paola Dugo Michele Manfra Pietro Campiglia |
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| Affiliation: | 1. Department of Pharmaceutical and Biomedical Sciences, University of Salerno, , Fisciano, (SA), Italy;2. Dipartimento di Chimica Farmaceutica e Tossicologica, Università di Napoli Federico II, , Napoli, Italy;3. Dipartimento di Scienze del farmaco e dei Prodotti per la Salute (SCIFAR), University of Messina, , Messina, Italy;4. Department of Science, University of Basilicata, , Potenza, Italy |
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| Abstract: | We have developed a fast ultra HPLC with ion‐trap TOF‐MS method for the analysis of flavonoids in Citrus bergamia juice. With respect to the typical methods for the analysis of these matrices based on conventional HPLC techniques, a tenfold faster separation was attained. The use of a core–shell particle column ensured high resolution within the fast analysis time of only 5 min. Unambiguous determination of flavonoid identity was obtained by the employment of a hybrid ion‐trap TOF mass spectrometer with high mass accuracy (average error 1.69 ppm). The system showed good retention time and peak area repeatability, with maximum RSD% values of 0.36 and 3.86, respectively, as well as good linearity (R2 ≥ 0.99). Our results show that ultra HPLC can be a useful tool for ultra fast qualitative/quantitative analysis of flavonoid compounds in citrus fruit juices. |
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| Keywords: | Bergamot Core‐Shell Citrus Flavonoids Mass accuracy |
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