1. Division of Systems and Engineering Management, School of Mechanical and Aerospace Engineering, Nanyang Technological Univeristy, 50 Nanyang Avenue, 639798 Singapore, Singapore;2. Division of Materials Technology, School of Materials Science and Engineering, Nanyang Technological Univerisity, 50 Nanyang Avenue, 639798 Singapore, Singapore;3. Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive, 117576 Singapore, Singapore;4. Experimental Fetal Medicine Group, Department of Obstetrics and Gynecology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, 119074 Singapore, Singapore;5. Department of Reproductive Medicine, KK Women's and Children's Hospital, 100 Bukit Timah Road, 229899, Singapore;6. Cancer and Stem Cell Biology Program, Duke‐NUS Graduate Medical School, Singapore;7. Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore, Singapore
Abstract:
Human mesenchymal stem cells (MSCs) derived from various origins show varied differentiation capability. Recent work shows that cell shape manipulation via micropatterning can modulate the differentiation of bone‐marrow‐derived MSCs. Herein, the effect of micropatterning on the myogenesis of MSCs isolated from three different sources (bone marrow, fetal tissue, and adipose) is reported. All the well‐aligned cells, regardless of source, predominantly commit to myogenic lineage, as shown by the significant upregulation of myogenic gene markers and positive myosin heavy chain staining. It is demonstrated that our novel micropattern can be used as a generic platform for inducing myogenesis of MSCs from different sources and may also have the potential to be extended to induce other lineage commitment.
