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Application of 13C isotope labeling using liquid chromatography mass spectrometry (LC‐MS) to determining phosphate‐containing metabolic incorporation |
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| Authors: | Ramakrishna Kommagani Sai Aparna Konde John P. Lydon Arun Sreekumar Nagireddy Putluri |
| Affiliation: | 1. Department of Molecular and Cell Biology, Baylor College of Medicine, , Houston, TX, 77030 USA;2. Alkek Center for Molecular Discovery, Baylor College of Medicine, , Houston, TX, 77030 USA;3. Verna and Marrs McLean Department of Biochemistry, Baylor College of Medicine, , Houston, TX, 77030 USA |
| Abstract: | Here, we describe an approach wherein negative electrospray ionization mass spectrometry has used to understand the relative flux through phosphate containing metabolic intermediates associated with central carbon metabolism after administering cells with 13C‐labeled substrates. The method was applied to examine the 13C incorporation through glycolysis in T47D breast cancer cells and showed reduction of glycolytic relative flux upon treatment with 2‐Deoxyglucose. Copyright © 2013 John Wiley & Sons, Ltd. |
| Keywords: | metabolic relative flux single reaction monitoring tandem mass spectrometry |