Preparative separation of flavonoids in plant extract of Smilacis Glabrae Roxb. by high performance counter‐current chromatography |
| |
Authors: | Huirong Zhang Bo Li Xiaofei Zong Li Li |
| |
Institution: | The College of Chemistry, Changchun Normal University, , China, Changchun |
| |
Abstract: | Four flavonoids, isoastilbin, astilbin, isoengelitin, and engelitin were isolated and purified simultaneously from Smilacis Glabrae Roxb. for the first time by high performance counter‐current chromatography using a system consisting of n‐hexane–n‐butanol–water (1:2:3, v/v/v). A total of 392.6 mg of astilbin, 71.4 mg of isoastilbin, 47.4 mg of engelitin, and 10.3 mg of isoengelitin were purified from 1.89 g of the ethyl acetate extract of Smilacis Glabrae Roxb. in six runs, each at over 94.51% purity as determined by HPLC. The structures of the four compounds were identified by their retention time, the LC‐ESI‐MSn in the negative ion mode, and confirmed by 1H‐NMR experiments. The characteristic LC‐ESI‐MS fragmentation patterns of the four compounds were discussed. |
| |
Keywords: | Flavonoids High performance counter‐current chromatography LC‐ESI‐MS Separation Smilacis Glabrae Roxb |
|
|